The rabbit anti-group sera against thirteen representative standard strains of Pseudomonas aeruginosa, A to M groups were prepared for preparation of serological diagnostic factor. Using the grouping sera for both slide and tube methods, the ag-glutination reaction has been carried out for each of the thirteen groups of viable and various killed cells of Pseudomonas aeruginosa, A to M groups as agglutinogens to obtain the following results,
1. Of the thirteen anti-group sera eleven showed highly specific antiseraa for serological typing on the slide agglutination regardless of kind of agglutinogens i.e. viable, thermostable, thermolabile or formalized cells.
2. When serological cross reaction of the standard group strains with each of the anti-sera was estimated by using agglutination reaction, the anti-G group serum showed 1+ to 2+ intensity of cross reaction with the viable cells of A, F, and: j grouping.,
3.The viable cells of F group were reacted with all anti-group sera except one.
4. The thermostable agglutinogen{120´c, 2hr) of group F strain gave no cross reac-tion with any of the thirteen anti-group serum.´ By contrast group M showed the cross reactivity with almost all of the anti-sera.
5. In the course of the immunization, of the thirteen groups, the agglutinin titers of each of anti-group sera, except for the thermolabile antigen were highest in two weeks after immunization by tube method.
6. Of the standard groups strains the group E was highly immunogenic. The anti-E serum showed agglutinin titer 2,560 times more than other groups for various ag-glutinogens as mentioned previously. The agglutinin titers of anti-C, -D, -I and -K group sera were shown even at 1,280 to 2,560 times dilution when thermostable antigens were used. In case of thermolabile antigens, however, the above titers were not appreciable at 20 times dilution.
7. In the titration of the agglutinins of 0 antigen of Pseudomonas aeruginosa, A to M groups, the thermostable antigens were most sensitive agglutinogen, while the thermolabile antigens were lowest in sensitivity.
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